Divergent allocation of sperm and the seminal proteome along a competition gradient in Drosophila melanogaster

Ejaculate quality plays an essential role in fertility, sperm competition, and offspring health. A key modulator of ejaculate quality is the social environment. Although males across taxa are known to strategically allocate sperm in response to rivals, how this applies to myriad other ejaculate components is poorly resolved. Here, we take a multilevel approach, from protein to fitness, to show that Drosophila melanogaster males divergently allocate sperm and seminal fluid proteins along a competition gradient. Using a combination of fluorescence-labeled sperm, quantitative proteomics, and multimating assays, we demonstrate that males are remarkably sensitive to the intensity of competition they perceive, show compositional change across and within portions of the ejaculate, and that this compositional change carries distinct costs and benefits.