Diverse gut bacteria communities protect against harmful pathogens by nutrient blocking

Bacteria in the mammalian gut. The bacteria are magenta, which are sitting above the gut wall. Host cell nuclei are the blue circles.

Image: Martin Jahn

The human gut is home to hundreds of different bacterial species collectively known as the gut microbiome. A major health benefit these provide is to protect the gut against invading pathogens (disease-causing microorganisms) that could cause harmful infections. But up to now, how this protective effect comes about has been unclear, and whether certain bacterial species have a more important role than others.

To investigate this, researchers at the University of Oxford tested 100 different gut bacteria strains individually and in combination for their ability to limit the growth of two harmful bacterial pathogens: Klebsiella pneumoniae and Salmonella enterica. Individual gut bacteria showed a very poor ability to restrict the spread of either pathogen. But when communities of up to 50 species were cultured together, the pathogens grew up to 1000 times less effectively than when cultured with any individual species. This ‘community protection effect’ was seen regardless of whether the bacteria were cultured together in vials, or in ‘germ-free’ mice (which had no resident gut bacteria at the start of the experiments). Professor Kevin Foster said:

"These results clearly demonstrate that colonisation resistance is a collective property of microbiome communities; in other words, a single strain is protective only when in combination with others."

Nutrient blocking: diverse gut bacteria consume the nutrients that a pathogen needs to invade and blocks it from the microbiome

Image: Erik Bakkeren

However, the researchers found that the members of the bacterial communities – and not just the overall diversity – had a critical effect on the level of protection. Certain species were found to be essential for community-based protection, even though these species offered little protection on their own.

The researchers demonstrated that protective bacterial communities block pathogen growth by consuming the nutrients that the pathogen needs. By assessing the genomes of the different bacterial species, they found that the most protective communities were composed of species with highly similar protein compositions to the pathogenic species. They also used metabolic profiling to demonstrate that the protective species had similar demands for carbon sources as the pathogens. Frances Spragge added:

"Crucially, although increased microbiome diversity increases the probability of protection against these pathogens, the overlap in nutrient utilisation profiles between the community and the pathogen is key. Certain species that have a crucial role in community protection show a high degree of metabolic overlap with the pathogen, and therefore similar nutrient demands."

The researchers used this nutrient blocking principle to predict communities of bacteria that would offer weak and strong protection against a different pathogen: an antimicrobial resistant E. coli strain. When tested experimentally, the communities which had the highest nutrient overlap with the E. coli strain were up to 100-fold more effective at reducing the pathogen’s abundance than the communities predicted to give weak protection.

According to the researchers, these new insights could be developed into new strategies to combat harmful gut pathogens through optimising gut microbiome communities. They may also explain why individuals can become more susceptible to species such as K. pneumoniae after taking antibiotic treatments that can lower the diversity of gut microbiome species. Dr Erik Bakkeren added:

"Our work supports the general hypothesis that a more diverse microbiome can carry health benefits. This gives promise to the goal of optimising the composition of microbiomes to protect against bacterial species that are harmful to health."

To read more about this research, published in Science, visit: http://www.science.org/doi/10.1126/science.adj3502